Enantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase

Enantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase

BACKGROUND Use of enzymes in low water media is now widely used for synthesis and kinetic resolution of organic compounds. The frequently used enzyme form is the freeze-dried powders. It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanol gives preparation (PREP) which show higher activity in low water media. The present work evaluates PREP of the lipas...

Immobilization of Rhizomucor miehei Lipase on High Density Polyethylene

 Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...

HYDROLYSIS OF FISH OIL BY HYPERACTIVATED Rhizomucor miehei IMMOBILIZED BY MULTIPOINT ANION EXCHANGE

STABILITY OF IMMOBILIZED Rhizomucor miehei LIPASE FOR THE SYNTHESIS OF PENTYL OCTANOATE IN A CONTINUOUS PACKED BED BIOREACTOR

The enzymatic synthesis of organic compounds in continuous bioreactors is an efficient way to obtain industrially important chemicals. However, few works have focused on the study of the operational conditions and the bioprocess performance. In this work, the aliphatic ester pentyl octanoate was obtained by direct esterification using a continuous packed bed bioreactor containing the immobilize...

Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids

To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257L...